Bio rad western blot all blue standards verification#
Bio rad western blot all blue standards plus#

That's why we're pleased to launch the color-enhanced Blu10 Plus (BLUltra) prestained protein standards.Īs you may see the comparison data shown below, Blu10 Plus (BLUltra) shows a better resolution than the Blu12 (BLUeye) & Blu10 (BlueRAY) for the protein bands from 95 kDa to 270 kDa. Having both unstained and prestained bands enables monitoring of electrophoresis, blot transfer and visualization of the standard after colorimetric or chemiluminescence development (when probed with StrepTactin-HRP or AP conjugate). Nowadays, more and more researchers are studying the high molecular weight proteins, meaning they may need high-resolution prestained protein standards, especially with high molecular weights. Precision Plus Protein WesternC standards these standards were designed for western blotting applications. Additional data should be considered for a more accurate adjustment. Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. For colorimetric or chemiluminescence blot detection, StrepTactin-HRP or -AP conjugate is also needed. Recombinant Precision Plus Protein Standards Natural Standards Protein standards for western blotting applications selection guide. Precision Plus Protein WesternC Standards Standard plugs are offered for 2-D electrophoresis applications: 1. Precision Plus Protein Prestained Standards 3. Precision Plus Protein Unstained Standards 2. 2.5 μl per well for general Western transferring. Bio-Rad's protein standards for western blotting applications. More Precision Plus Protein Standards: 1. Migration patterns of Blu10 Plus Prestained Protein Ladder (BLUltra) in different electrophoresis conditions are listed below :ģ μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. Guide for Molecular Weight Estimation (kDa) The Blu10 Plus Prestained Protein Ladder (BLUltra) keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes. V3Western Workflow Save time, identify problems earlier, and have more confidence in your western blot results. The 10 recombinant proteins are covalently coupled with blue chromophore, while 2 orange bands at 30 kDa and 270 kDa and a green band at 52 kDa serve as reference bands. Improved Dual Color Standards are brighter than the competition on both gels and blots.The Blu10 Plus Prestained Protein Ladder (BLUltra) is a combination of 10 pre-stained proteins with molecular weights from 6.5 to 270 kDa. Increased overall brightness provides visual robustness throughout the entire protein electrophoresis and western blotting workflow.

Bio rad western blot all blue standards verification#

Applications include verification of Western transfer efficiency on. 8 blue-stained bands and 2 pink reference bands (25 and 75 kD) Allows approximate molecular weight determination when performing SDS-PAGE analysis.Mixture of 10 recombinant proteins of precise molecular weights (10–250 kD).Use improved Precision Plus Protein Dual Color Standards for molecular weight estimation on SDS-PAGE gels and western blots. Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple. LC-MS innovation: Improve analytical accuracy Which microbioreactor modules are best for you Not to be confused with fluorescent total blot stains. Back to Top Applications of Bio-Rad's Protein Standards Applications of Bio-Rad's protein standards. full-length proteins, whereas a western blot of a Bio-Rad TGX gel shows multiple. Details of Bio-Rad's protein standards are provided in the tables and figures below. Which solid support is right for your oligo synthesis? For a complete listing of all available protein gel electrophoresis. To specify how molecular weight is analyzed. Lab Automation, AI & Digital Transformation See Appendix D, Accessories for all Bio-Rad standards and their catalog numbers.Food Contamination: Quality, Safety & Authenticity.